Trans-9,Trans-11
Conjugated Linoleic Acid Decreases Triglyceride Accumulation in Primary
Cultures of Pre-Adipocytes from Human and Hamster Adipose Tissues
V.C. Gavino*, G.G. Gavino & R.-M. Pelletier
Dèpartement de Nutrition, Universitè de Montrèal,
Montrèal QC
CLA
preparations exhibit several biological effects in various experimental
models. This pleiotropic behaviour may be partly due to proportions of
specific CLA isomers found in commercial preparations, given that each
particular isomer may have unique biological properties relative to the
other forms. Since it has been shown that CLA preparations favorably modulate
lipid metabolism in mammals, we tested specific CLA isomers for their
ability to influence lipid-filling in primary cultures of stromal-vascular
cells from human and hamster adipose tissues. One such isomer is the trans-9,trans-11
form, present in milkfat and in commercial CLA preparations at approximately
10% and 6.5% of total CLA, respectively. We exposed cells to 50(M concentrations
of various pure CLA isomers using linoleic acid as control. Extent of
differentiation, measured by induction of cellular glycerol-3-phosphate
dehydrogenase, was the same for all groups. The trans-9,trans-11 isomer
compared to linoleic acid, lowered lipid-filling by 85% and 45% in human
and hamster pre-adipocytes, respectively. In contrast, the trans-10,cis-12
isomer decreased lipid-filling in human cells by 30%, and had no effect
on hamster cells compared to control. We conclude that the trans-9,trans-11
isomer may contribute to the overall effect of CLA preparations on body
fat.
Funded by the Dairy Farmers of Canada and by NSERC.
